Immunolocalization of Acyl-Coenzyme A:CholesterolO-Acyltransferase in Macrophages
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چکیده
منابع مشابه
Acyl-coenzyme A:cholesterol acyltransferase promotes oxidized LDL/oxysterol-induced apoptosis in macrophages.
7-Ketocholesterol (7KC) is a cytotoxic component of oxidized low density lipoproteins (OxLDLs) and induces apoptosis in macrophages by a mechanism involving the activation of cytosolic phospholipase A2 (cPLA2). In the current study, we examined the role of ACAT in 7KC-induced and OxLDL-induced apoptosis in murine macrophages. An ACAT inhibitor, Sandoz 58-035, suppressed 7KC-induced apoptosis in...
متن کاملAcyl-coenzyme A--cholesterol acyltransferase activity in human liver.
1. In the presence of CoA and ATP, human liver microsomes catalyse the incorporation of [14C]oleate or [14C]cholesterol into cholesteryl oleate, thus demonstrating the presence of acyl-coenzyme A-cholesterol acyltransferase (cholesterol acyltransferase) in human liver. 2. The enzyme has properties similar to those of rat liver enzyme and with both the concentration of endogenous cholesterol in ...
متن کاملRoles of acyl-coenzyme A:cholesterol acyltransferase-1 and -2.
Acyl-coenzyme A:cholesterol acyltransferase (ACAT) is an intracellular enzyme that produces cholesteryl esters in various tissues. In mammals, two ACAT genes (ACAT1 and ACAT2) have been identified. Together, these two enzymes are involved in storing cholesteryl esters as lipid droplets, in macrophage foam-cell formation, in absorbing dietary cholesterol, and in supplying cholesteryl esters as p...
متن کاملAt4g24160, a soluble acyl-coenzyme A-dependent lysophosphatidic acid acyltransferase.
Human CGI-58 (for comparative gene identification-58) and YLR099c, encoding Ict1p in Saccharomyces cerevisiae, have recently been identified as acyl-CoA-dependent lysophosphatidic acid acyltransferases. Sequence database searches for CGI-58 like proteins in Arabidopsis (Arabidopsis thaliana) revealed 24 proteins with At4g24160, a member of the alpha/beta-hydrolase family of proteins being the c...
متن کاملAcyl coenzyme A:2-acyl-sn-glycerol-3-phosphate acyltransferase activity in rat liver microsomes.
z-Acylglycerol-3-phosphate can be acylated by acyl-CoA in the presence of microsomal preparations from rat liver. With optimal amounts of substrate, the acyltransferase reaction to the r-position occurred at about one tenth the rate observed for the z-position. Oleate was esterified more rapidly than palmitate or stearate under the conditions used. The enzymic activity catalyzing esterification...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1998
ISSN: 0021-9258
DOI: 10.1074/jbc.273.18.11218